An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of a Regulatory Subunit of Protein Phosphatase 1 Which Mediates Blue Light Signaling for Stomatal Opening.

Takemiya A, Yamauchi S, Yano T, Ariyoshi C, Shimazaki KI

Protein phosphatase 1 (PP1) is a eukaryotic Ser/Thr protein phosphatase comprised of a catalytic subunit (PP1c) and a regulatory subunit that modulates catalytic activity, subcellular localization, and substrate specificity. PP1c positively regulates stomatal opening through blue light signaling between phototropins and the plasma membrane H(+)-ATPase in guard cells. However, the regulatory subunit functioning in this process is unknown. We identified ... [more]

Unknown May. 13, 2012; 0(0); [Pubmed: 22585556]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TOPP4 GEM	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Takemiya A (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TOPP4

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein serine/threonine phosphatase activity [IDA, ISS]

Gene Ontology Cellular Component

GEM