BAIT

HTT

HD, IT15
huntingtin
Alzheimer's Disease Project
Homo sapiens
PREY

DCN1

NEDD8 ligase DCN1, YLR128W
Scaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation
UPS Project
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Yeast genes that enhance the toxicity of a mutant huntingtin fragment or alpha-synuclein.

Willingham S, Outeiro TF, DeVit MJ, Lindquist SL, Muchowski PJ

Genome-wide screens were performed in yeast to identify genes that enhance the toxicity of a mutant huntingtin fragment or of alpha-synuclein. Of 4850 haploid mutants containing deletions of nonessential genes, 52 were identified that were sensitive to a mutant huntingtin fragment, 86 that were sensitive to alpha-synuclein, and only one mutant that was sensitive to both. Genes that enhanced toxicity ... [more]

Science Dec. 05, 2003; 302(5651);1769-72 [Pubmed: 14657499]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • deletion of the yeast gene enhances toxicity of the human huntington HD53Q allele in a yeast background

Curated By

  • BioGRID