BAIT

SLC6A4

5-HTT, AI323329, Htt, Sert, RP23-100P23.1
solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation.

Mouri A, Sasaki A, Watanabe K, Sogawa C, Kitayama S, Mamiya T, Miyamoto Y, Yamada K, Noda Y, Nabeshima T

The ubiquitin-proteasome system (UPS) controls the stability of most cellular proteins. The polymorphism of UPS-related genes is associated with major depression disorder, but less is known about the molecule that plays a role in depression by modulating the UPS. Melanoma antigen gene-D1 (MAGE-D1) interacts with RING E3 ubiquitin ligase and is implicated in protein degradation. MAGE-D1 may thus play an ... [more]

J. Neurosci. Mar. 28, 2012; 32(13);4562-80 [Pubmed: 22457503]

Throughput

  • Low Throughput

Curated By

  • BioGRID