An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily.

Kutay U, Hartmann E, Treichel N, Calado A, Carmo-Fonseca M, Prehn S, Kraft R, Gorlich D, Bischoff FR

Nucleo-cytoplasmic transport comprises a large number of distinct pathways, many of which are defined by members of the importin beta superfamily of nuclear transport receptors. These transport receptors all directly interact with RanGTP to modulate the compartment-specific binding of their transport substrates. To identify new members of the importin beta family, we used affinity chromatography on immobilized RanGTP and isolated ... [more]

J. Biol. Chem. Dec. 22, 2000; 275(51);40163-8 [Pubmed: 11024021]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAN XPO7	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kutay U (2000)	Low	-	BioGRID	-
XPO7 RAN	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2722211

Curated By

BioGRID

Interaction Summary

RAN

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IDA]GTPase activity [TAS]androgen receptor binding [NAS]chromatin binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]transcription coactivator activity [NAS]

Gene Ontology Cellular Component

XPO7