An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic identification of the MYST domain histone acetyltransferase TIP60 (HTATIP) as a co-activator of the myeloid transcription factor C/EBPalpha.

Bararia D, Trivedi AK, Zada AA, Greif PA, Mulaw MA, Christopeit M, Hiddemann W, Bohlander SK, Behre G

The transcription factor C/EBPalpha (CEBPA) is a key player in granulopoiesis and leukemogenesis. We have previously reported the interaction of C/EBPalpha with other proteins (utilizing mass spectrometry) in transcriptional regulation. In the present study, we characterized the association of the MYST domain histone acetyltransferase Tat-interactive protein (TIP) 60 (HTATIP) with C/EBPalpha. We show in pull-down and co-precipitation experiments that C/EBPalpha ... [more]

Leukemia Apr. 01, 2008; 22(4);800-7 [Pubmed: 18239623]

Throughput

High Throughput

Additional Notes

figure 1 and supplementary table 1.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CEBPA GRIN3A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Zada AA (2006)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CEBPA

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [NAS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA]transcription factor binding [IPI]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

GRIN3A

Gene Ontology Biological Process

Gene Ontology Molecular Function

N-methyl-D-aspartate selective glutamate receptor activity [IDA, ISS]extracellular-glutamate-gated ion channel activity [IBA]glycine binding [IDA]identical protein binding [IPI]protein binding [IPI]protein phosphatase 2A binding [ISS]