Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Doubling the size of the glucocorticoid receptor ligand binding pocket by deacylcortivazol.

Suino-Powell K, Xu Y, Zhang C, Tao YG, Tolbert WD, Simons SS, Xu HE

A common feature of nuclear receptor ligand binding domains (LBD) is a helical sandwich fold that nests a ligand binding pocket within the bottom half of the domain. Here we report that the ligand pocket of glucocorticoid receptor (GR) can be continuously extended into the top half of the LBD by binding to deacylcortivazol (DAC), an extremely potent glucocorticoid. It ... [more]

Mol. Cell. Biol. Mar. 01, 2008; 28(6);1915-23 [Pubmed: 18160712]

Throughput

  • Low Throughput

Additional Notes

  • figure 2B. each peptide contains 15 residues.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NR3C1 NR0B1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
2447731

Curated By

  • BioGRID