SET2
Gene Ontology Biological Process
Gene Ontology Molecular Function
ELL
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
MSL complex is attracted to genes marked by H3K36 trimethylation using a sequence-independent mechanism.
In Drosophila, X chromosome dosage compensation requires the male-specific lethal (MSL) complex, which associates with actively transcribed genes on the single male X chromosome to upregulate transcription approximately 2-fold. We found that on the male X chromosome, or when MSL complex is ectopically localized to an autosome, histone H3K36 trimethylation (H3K36me3) is a strong predictor of MSL binding. We isolated ... [more]
Throughput
- Low Throughput
Additional Notes
- figure S5. The C-terminal domain of CG1716 associates specifically with the phosphorylated Pol II CTD (Ser2P or Ser5P).
Curated By
- BioGRID