An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Physical and functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR, a polycomb-like protein.

Lee MG, Norman J, Shilatifard A, Shiekhattar R

Histone methylation is a posttranslational modification regulating chromatin structure and gene regulation. BHC110/LSD1 was the first histone demethylase described to reverse dimethyl histone H3 lysine 4 (H3K4). Here we show that JARID1d, a JmjC-domain-containing protein, specifically demethylates trimethyl H3K4. Detailed mapping analysis revealed that besides the JmjC domain, the BRIGHT and zinc-finger-like C(5)HC(2) domains are required for maximum catalytic activity. ... [more]

Cell Mar. 09, 2007; 128(5);877-87 [Pubmed: 17320162]

Throughput

Low Throughput

Additional Notes

figure 1C.
figure 2. Jarid1d specifically demethylates trimethylated H3K4. BRIGHT and Zinc Finger like C5HC2 domains contribute to the catalytic activity.
figure 4. demethylation activity of Jarid1d is enhanced by the addition of MBLR protein.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KDM5D HIST3H3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lee MG (2007)	Low	-	BioGRID	658297
KDM5D HIST3H3	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Lee MG (2007)	Low	-	BioGRID	658298

Curated By

BioGRID

Interaction Summary

KDM5D

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone demethylase activity (H3-K4 specific) [IDA]

HIST3H3