WRN
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA duplex unwinding [IDA, IMP]
- DNA metabolic process [IDA]
- DNA replication [IMP]
- DNA synthesis involved in DNA repair [IDA]
- aging [NAS]
- base-excision repair [IDA]
- cell aging [IMP]
- cellular response to DNA damage stimulus [IDA]
- cellular response to gamma radiation [IDA]
- cellular response to starvation [IDA]
- double-strand break repair [IMP]
- multicellular organismal aging [IMP]
- nucleic acid phosphodiester bond hydrolysis [IDA]
- nucleolus to nucleoplasm transport [IDA]
- positive regulation of hydrolase activity [IDA]
- regulation of apoptotic process [IGI]
- replication fork processing [IDA, IMP]
- response to UV-C [IDA]
- response to oxidative stress [IDA]
- telomere maintenance [IMP]
Gene Ontology Molecular Function- 3'-5' DNA helicase activity [IDA]
- 3'-5' exonuclease activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- DNA helicase activity [IDA, IMP]
- G-quadruplex DNA binding [IDA]
- Y-form DNA binding [IDA]
- bubble DNA binding [IDA]
- exonuclease activity [IDA]
- four-way junction helicase activity [IDA]
- helicase activity [IDA]
- magnesium ion binding [IDA]
- manganese ion binding [IDA]
- protein binding [IPI]
- protein complex binding [IDA]
- protein homodimerization activity [IDA]
- 3'-5' DNA helicase activity [IDA]
- 3'-5' exonuclease activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- DNA helicase activity [IDA, IMP]
- G-quadruplex DNA binding [IDA]
- Y-form DNA binding [IDA]
- bubble DNA binding [IDA]
- exonuclease activity [IDA]
- four-way junction helicase activity [IDA]
- helicase activity [IDA]
- magnesium ion binding [IDA]
- manganese ion binding [IDA]
- protein binding [IPI]
- protein complex binding [IDA]
- protein homodimerization activity [IDA]
Gene Ontology Cellular Component
PARP1
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to insulin stimulus [IDA]
- double-strand break repair [IMP]
- gene expression [TAS]
- macrophage differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- protein ADP-ribosylation [IDA]
- protein poly-ADP-ribosylation [IDA]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1.
Werner's syndrome (WS) is an inherited disease characterized by genomic instability and premature aging. The WS gene encodes a protein (WRN) with helicase and exonuclease activities. We have previously reported that WRN interacts with Ku70/80 and this interaction strongly stimulates WRN exonuclease activity. To gain further insight on the function of WRN and its relationship with the Ku heterodimer, we ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WRN PARP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| PARP1 WRN | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID