AKAP9
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- cellular response to cAMP [IMP]
- mitotic cell cycle [TAS]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of potassium ion transmembrane transporter activity [IMP]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of membrane repolarization [IMP]
- regulation of ventricular cardiac muscle cell membrane repolarization [IMP]
- synaptic transmission [TAS]
- transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CCNE1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Centrosome-targeting region of CG-NAP causes centrosome amplification by recruiting cyclin E-cdk2 complex.
Centrosome duplication occurs once per cell cycle and is thought to be triggered by cyclin E-cdk2. However, it is largely unknown how the duplication is regulated. Here, we found that the expression of the centrosome-targeting region of CG-NAP (centrosome and Golgi-localized PKN-associated protein), which we designate as CG-NAP/D, increased the number of centrosomes in Chinese hamster ovary (CHO)-K1 cells. The ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 5A.
- figure 5B. full length of CG-NAP.
- figure 9B. NLS along with R145 is not required for the association.
Curated By
- BioGRID