Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Dissection of amyloid-beta precursor protein-dependent transcriptional transactivation.

Cao X, Suedhof TC

Amyloid-beta precursor protein (APP) forms a transcriptionally active complex with the adaptor protein Fe65 and the histone acetyltransferase Tip60, but the mechanism of transcriptional activation that is mediated by APP and Fe65 remains unclear. APP is cleaved by gamma-secretase similar to Notch, whose intracellular domain activates transcription by interacting with nuclear transcription factors. To test whether the APP intracellular domain ... [more]

J. Biol. Chem. Jun. 04, 2004; 279(23);24601-11 [Pubmed: 15044485]

Throughput

  • Low Throughput

Additional Notes

  • figure 8. Interaction between the WW and PTB2 domains of Fe65.

Curated By

  • BioGRID