An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis.

Cavusoglu N, Brand M, Tora L, Van Dorsselaer A

Initiation of transcription of protein-encoding genes by RNA polymerase II was thought to require the transcription factor II D (TF(II)D), a complex comprising the TATA binding protein (TBP) and TBP-associated factors. However, another multiprotein complex isolated more recently and called TFTC (TBP-free TAF(II )containing complex), was shown to mediate initiation of RNA polymerase II (Pol II) transcription in the absence ... [more]

Proteomics Feb. 01, 2003; 3(2);217-23 [Pubmed: 12601814]

Throughput

Low Throughput

Additional Notes

figure 1, table 1.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TAF10 TAF5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.9993	BioGRID	3271713
TAF10 TAF5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Nagy Z (2009)	High	-	BioGRID	-
TAF10 TAF5	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2012)	High	0.839	BioGRID	740752
TAF10 TAF5	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Demeny MA (2007)	Low	-	BioGRID	-
TAF10 TAF5	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Wieczorek E (1998)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TAF10

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase binding [IDA]enzyme binding [IPI]estrogen receptor binding [IDA]histone acetyltransferase activity [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA]transcription coactivator activity [IDA]

Gene Ontology Cellular Component

TAF5

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone acetyltransferase activity [IDA]protein binding [IPI]protein dimerization activity [IDA]sequence-specific DNA binding transcription factor activity [IDA, IMP]transcription regulatory region DNA binding [IDA]