BAIT

PST2

YDR032C
Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
GO Process (0)
GO Function (0)
GO Component (4)

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

FTR1

high-affinity iron permease FTR1, L000003066, YER145C
High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Multiplex assay for condition-dependent changes in protein-protein interactions.

Schlecht U, Miranda M, Suresh S, Davis RW, St Onge RP

Changes in protein-protein interactions that occur in response to environmental cues are difficult to uncover and have been poorly characterized to date. Here we describe a yeast-based assay that allows many binary protein interactions to be assessed in parallel and under various conditions. This method combines molecular bar-coding and tag array technology with the murine dihydrofolate reductase-based protein-fragment complementation assay. ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 05, 2012; 109(23);9213-8 [Pubmed: 22615397]

Throughput

  • High Throughput

Additional Notes

  • interaction determined by murine dihydrofolate reductase-based protein-fragment complementation assay (mDHFR PCA)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PST2 FTR1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1341BioGRID
2093403
PST2 FTR1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
-

Curated By

  • BioGRID