ITSN1
Gene Ontology Biological Process
- apoptotic signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of GTPase activity [NAS]
- positive regulation of apoptotic process [TAS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- synaptic vesicle endocytosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ITSN2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Intersectin (ITSN) Family of Scaffolds Function as Molecular Hubs in Protein Interaction Networks.
Members of the intersectin (ITSN) family of scaffold proteins consist of multiple modular domains, each with distinct ligand preferences. Although ITSNs were initially implicated in the regulation of endocytosis, subsequent studies have revealed a more complex role for these scaffold proteins in regulation of additional biochemical pathways. In this study, we performed a high throughput yeast two-hybrid screen to identify ... [more]
Throughput
- Low Throughput
Additional Notes
- BiFC
Curated By
- BioGRID