An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Intersectin (ITSN) Family of Scaffolds Function as Molecular Hubs in Protein Interaction Networks.

Wong KA, Wilson J, Russo A, Wang L, Okur MN, Wang X, Martin NP, Scappini E, Carnegie GK, O'Bryan JP

Members of the intersectin (ITSN) family of scaffold proteins consist of multiple modular domains, each with distinct ligand preferences. Although ITSNs were initially implicated in the regulation of endocytosis, subsequent studies have revealed a more complex role for these scaffold proteins in regulation of additional biochemical pathways. In this study, we performed a high throughput yeast two-hybrid screen to identify ... [more]

PLoS ONE May. 05, 2012; 7(4);e36023 [Pubmed: 22558309]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DISC1 ITSN1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Morris JA (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ITSN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [NAS]guanyl-nucleotide exchange factor activity [NAS]proline-rich region binding [IPI]protein binding [IPI]protein complex scaffold [IDA]

Gene Ontology Cellular Component

DISC1