An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Meiotic expression of the cyclin H/Cdk7 complex in male germ cells of the mouse.

Kim JM, McGaughy JT, Bogle RK, Ravnik SE

Cell division requires that cyclin-dependent kinases (Cdks) be activated by phosphorylation. In mitotic cells, this is accomplished by the Cdk-activating-kinase (CAK), which is a complex of cyclin H and Cdk7. There are currently no data on the role of CAK in meiotic cells. Previously, we have shown that cyclin A1 is meiosis-specific and forms an active kinase with Cdk2. Because ... [more]

Biol. Reprod. May. 01, 2001; 64(5);1400-8 [Pubmed: 11319144]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CCNH CDK7	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Kim JM (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDK7

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-dependent ATPase activity [ISO]RNA polymerase II carboxy-terminal domain kinase activity [ISO]cyclin-dependent protein serine/threonine kinase activity [ISO, ISS]kinase activity [IDA]protein C-terminus binding [ISO]protein binding [IPI]protein complex binding [ISO]protein kinase activity [IDA]

Gene Ontology Cellular Component

CCNH