An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Ubiquitin-mediated proteolysis of HuR by heat shock.

Abdelmohsen K, Srikantan S, Yang X, Lal A, Kim HH, Kuwano Y, Galban S, Becker KG, Kamara D, de Cabo R, Gorospe M

The RNA-binding protein HuR regulates the stability and translation of numerous mRNAs encoding stress-response and proliferative proteins. Although its post-transcriptional influence has been linked primarily to its cytoplasmic translocation, here we report that moderate heat shock (HS) potently reduces HuR levels, thereby altering the expression of HuR target mRNAs. HS did not change HuR mRNA levels or de novo translation, ... [more]

EMBO J. May. 06, 2009; 28(9);1271-82 [Pubmed: 19322201]

Throughput

High Throughput

Ontology Terms

hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ELAVL1 CELF1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.0884	BioGRID	1259908
ELAVL1 CELF1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	High	-	BioGRID	2813499
CELF1 ELAVL1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Youn JY (2018)	Low/High	-	BioGRID	2812632

Curated By

BioGRID

Interaction Summary

ELAVL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

AU-rich element binding [IDA]RNA binding [IDA]double-stranded RNA binding [IDA]mRNA 3'-UTR AU-rich region binding [IDA]mRNA 3'-UTR binding [ISS]mRNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]

Gene Ontology Cellular Component

CELF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

BRE binding [IDA]RNA binding [IDA]mRNA binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]translation repressor activity, nucleic acid binding [NAS]