An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Ubiquitin-mediated proteolysis of HuR by heat shock.

Abdelmohsen K, Srikantan S, Yang X, Lal A, Kim HH, Kuwano Y, Galban S, Becker KG, Kamara D, de Cabo R, Gorospe M

The RNA-binding protein HuR regulates the stability and translation of numerous mRNAs encoding stress-response and proliferative proteins. Although its post-transcriptional influence has been linked primarily to its cytoplasmic translocation, here we report that moderate heat shock (HS) potently reduces HuR levels, thereby altering the expression of HuR target mRNAs. HS did not change HuR mRNA levels or de novo translation, ... [more]

EMBO J. May. 06, 2009; 28(9);1271-82 [Pubmed: 19322201]

Throughput

High Throughput

Ontology Terms

cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CHD4 ELAVL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hoffmeister H (2017)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ELAVL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

AU-rich element binding [IDA]RNA binding [IDA]double-stranded RNA binding [IDA]mRNA 3'-UTR AU-rich region binding [IDA]mRNA 3'-UTR binding [ISS]mRNA binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]

Gene Ontology Cellular Component

CHD4