Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Predicting the fission yeast protein interaction network.

Pancaldi V, Sarac OS, Rallis C, McLean JR, Prevorovsky M, Gould K, Beyer A, Baehler J

A systems-level understanding of biological processes and information flow requires the mapping of cellular component interactions, among which protein-protein interactions are particularly important. Fission yeast (Schizosaccharomyces pombe) is a valuable model organism for which no systematic protein-interaction data are available. We exploited gene and protein properties, global genome regulation datasets, and conservation of interactions between budding and fission yeast to ... [more]

G3 (Bethesda) Apr. 01, 2012; 2(4);453-67 [Pubmed: 22540037]

Throughput

  • High Throughput

Additional Notes

  • file S4.

Curated By

  • BioGRID