BAIT

UBC13

E2 ubiquitin-conjugating protein UBC13, L000003387, YDR092W
E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
UPS Project
GO Process (4)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MPH1

YIR002C
3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA in error-free bypass pathway, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; similar to FANCM human Fanconi anemia complementation group protein that with MHF complex is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Rad5-dependent DNA repair functions of the Saccharomyces cerevisiae FANCM homolog Mph1.

Daee DL, Ferrari E, Longerich S, Zheng XF, Xue X, Branzei D, Sung P, Myung K

Interstrand crosslinks (ICLs) covalently link complementary DNA strands, block DNA replication and transcription, and must be removed to allow cell survival. Several pathways, including the Fanconi Anemia (FA) pathway, can faithfully repair ICLs and maintain genomic integrity; however the precise mechanisms of most ICL repair processes remain enigmatic. In this study, we genetically characterized a conserved yeast ICL repair pathway ... [more]

Unknown Jun. 12, 2012; 0(0); [Pubmed: 22696213]

Throughput

  • Low Throughput

Ontology Terms

  • resistance to chemicals (APO:0000087)
  • vegetative growth (APO:0000106)

Additional Notes

  • overexpression of mph1 decreases sensitivity to NM (CHEBI 37598 CID 4033)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBC13 MPH1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2147BioGRID
2094624

Curated By

  • BioGRID