BAIT

SF3A2

PRP11, PRPF11, SAP62, SF3a66
splicing factor 3a, subunit 2, 66kDa
Homo sapiens
PREY

HNRNPA3

2610510D13Rik, D10S102, FBRNP, HNRPA3
heterogeneous nuclear ribonucleoprotein A3
GO Process (3)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.

Behzadnia N, Golas MM, Hartmuth K, Sander B, Kastner B, Deckert J, Dube P, Will CL, Urlaub H, Stark H, Luehrmann R

Little is known about the higher-order structure of prespliceosomal A complexes, in which pairing of the pre-mRNA's splice sites occurs. Here, human A complexes were isolated under physiological conditions by double-affinity selection. Purified complexes contained stoichiometric amounts of U1, U2 and pre-mRNA, and crosslinking studies indicated that these form concomitant base pairing interactions with one another. A complexes contained nearly ... [more]

EMBO J. Mar. 21, 2007; 26(6);1737-48 [Pubmed: 17332742]

Throughput

  • Low Throughput

Curated By

  • BioGRID