A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Xyloglucan xylosyltransferases XXT1, XXT2, XXT5, and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes.

Chou YH, Pogorelko G, Zabotina O

Xyloglucan is the major hemicellulosic polysaccharide in the primary cell walls of most vascular dicotyledonous plants, and has important structural and physiological functions in plant growth and development. In Arabidopsis thaliana, the 1,4-Î²-glucan synthase CSLC4 and three xylosyltransferases, XXT1, XXT2, and XXT5, act in Golgi to form the xylosylated glucan backbone during xyloglucan biosynthesis. However, the functional organization of these ... [more]

Unknown Jun. 04, 2012; 0(0); [Pubmed: 22665445]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
XT1 XT2	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Lund CH (2015)	Low	-	BioGRID	1111971
XT2 XT1	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Lund CH (2015)	Low	-	BioGRID	1111975

Curated By

BioGRID

Interaction Summary

XT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

UDP-xylosyltransferase activity [IDA]protein binding [IPI]transferase activity [ISS]transferase activity, transferring glycosyl groups [ISS]xyloglucan 6-xylosyltransferase activity [IDA]

Gene Ontology Cellular Component

XT2