INN1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SLA1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Sho1p connects the plasma membrane with proteins of the cytokinesis network via multiple isomeric interaction states.
A molecular understanding of cytokinesis requires the detailed description of the protein complexes that perform central activities during this process. The proteins Hof1p, Cyk3p, Inn1p, and Myo1p each represent one of the four genetically defined and partially complementing pathways of cytokinesis in the yeast Saccharomyces cerevisiae. Here we show that the osmosensor Sho1p is required for correct cell-cell separation. Shortly ... [more]
Throughput
- Low Throughput
Additional Notes
- split-ubiquitin
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
INN1 SLA1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.56 | BioGRID | 2009780 | |
SLA1 INN1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID