BAIT

SHFM1

Shfdg1, wu:fb55c02, zgc:56203, zgc:85901
split hand/foot malformation (ectrodactyly) type 1
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Danio rerio
PREY

PSMD3

zgc:65867, zgc:77369
proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
GO Process (0)
GO Function (0)
GO Component (0)
Danio rerio

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of a specific motif of the DSS1 protein required for proteasome interaction and p53 protein degradation.

Wei SJ, Williams JG, Dang H, Darden TA, Betz BL, Humble MM, Chang FM, Trempus CS, Johnson K, Cannon RE, Tennant RW

Deleted in Split hand/Split foot 1 (DSS1) was previously identified as a novel 12-O-tetradecanoylphorbol-13-acetate (TPA)-inducible gene with possible involvement in early event of mouse skin carcinogenesis. The mechanisms by which human DSS1 (HsDSS1) exerts its biological effects via regulation of the ubiquitin-proteasome system (UPS) are currently unknown. Here, we demonstrated that HsDSS1 regulates the human proteasome by associating with it ... [more]

J. Mol. Biol. Nov. 14, 2008; 383(3);693-712 [Pubmed: 18775730]

Throughput

  • Low Throughput

Curated By

  • BioGRID