BAIT

PSF2

bsh3, dre13, SPBC725.13c
GINS complex subunit Psf2
GO Process (3)
GO Function (0)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

PSF1

SPBP23A10.09
GINS complex subunit Psf1
GO Process (2)
GO Function (0)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T, Kanke M, Takahashi TS, Nakagawa T, Masukata H

DNA polymerase epsilon (Pol ε) synthesizes the leading strands, following the CMG (Cdc45/Mcm2-7/GINS) helicase that translocates on the leading strand template at eukaryotic replication forks. Although Pol ε is essential for the viability of fission and budding yeasts, the N-terminal polymerase domain of the catalytic subunit, Cdc20/Pol2, is dispensable for viability, leaving a question what is the essential role(s) of ... [more]

Unknown Jun. 20, 2012; 0(0); [Pubmed: 22718908]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PSF2 PSF1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID