BAIT

RPN501

rpn5, rpn5-a, SPAC1420.03
19S proteasome regulatory subunit Rpn501
Schizosaccharomyces pombe (972h)
PREY

RPN11

bfr2, mts5, pad1, sks1, SPAC31G5.13
19S proteasome regulatory subunit Rpn11
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly.

Yen HC, Espiritu C, Chang EC

Proper function of the 26 S proteasome requires assembly of the regulatory complex, which is composed of the lid and base subcomplexes. We characterized Rpn5, a lid subunit, in fission yeast. We show that Rpn5 associates with the proteasome rpn5. Deletion (rpn5Delta) exacerbates the growth defects in proteasome mutants, leading to mitotic abnormalities, which correlate with accumulation of polyubiquitinated proteins, ... [more]

J. Biol. Chem. Aug. 15, 2003; 278(33);30669-76 [Pubmed: 12783882]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • rpn5/pad1 mutants are lethal

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPN11 RPN501
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID