BAIT

RPN502

rpn5, rpn5-b, SPAPB8E5.02c
19S proteasome regulatory subunit Rpn502
Schizosaccharomyces pombe (972h)
PREY

RPN11

bfr2, mts5, pad1, sks1, SPAC31G5.13
19S proteasome regulatory subunit Rpn11
GO Process (3)
GO Function (1)
GO Component (4)
Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly.

Yen HC, Espiritu C, Chang EC

Proper function of the 26 S proteasome requires assembly of the regulatory complex, which is composed of the lid and base subcomplexes. We characterized Rpn5, a lid subunit, in fission yeast. We show that Rpn5 associates with the proteasome rpn5. Deletion (rpn5Delta) exacerbates the growth defects in proteasome mutants, leading to mitotic abnormalities, which correlate with accumulation of polyubiquitinated proteins, ... [more]

J. Biol. Chem. Aug. 15, 2003; 278(33);30669-76 [Pubmed: 12783882]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • rpn5/pad1 mutants are lethal

Curated By

  • BioGRID