BAIT
BHLHE40
Bhlhb2, C130042M06Rik, CR8, Clast5, Dec1, Sharp2, Stra13, Stra14
basic helix-loop-helix family, member e40
GO Process (8)
GO Function (14)
GO Component (4)
Gene Ontology Biological Process
- circadian regulation of gene expression [IMP, ISO]
- entrainment of circadian clock [ISO]
- entrainment of circadian clock by photoperiod [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- response to light stimulus [ISO]
- transcription from RNA polymerase II promoter [IDA, ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- E-box binding [IDA, ISO]
- MRF binding [IDA]
- RNA polymerase II activating transcription factor binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- bHLH transcription factor binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein heterodimerization activity [IDA, ISO]
- protein homodimerization activity [ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- transcription corepressor activity [IDA, ISO]
- DNA binding [IDA]
- E-box binding [IDA, ISO]
- MRF binding [IDA]
- RNA polymerase II activating transcription factor binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [ISO]
- bHLH transcription factor binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein heterodimerization activity [IDA, ISO]
- protein homodimerization activity [ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- transcription corepressor activity [IDA, ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
HDAC1
HD1, Hdac1-ps, MommeD5, RPD3, RP23-209C6.7
histone deacetylase 1
GO Process (42)
GO Function (24)
GO Component (16)
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [ISO]
- cellular response to oxidative stress [ISO]
- chromatin modification [TAS]
- circadian regulation of gene expression [IDA]
- circadian rhythm [IDA]
- embryonic digit morphogenesis [IGI]
- endoderm development [IDA, IMP]
- epidermal cell differentiation [IGI]
- eyelid development in camera-type eye [IGI]
- fungiform papilla formation [IGI]
- hair follicle placode formation [IGI]
- hippocampus development [IGI]
- histone H3 deacetylation [IDA, ISO]
- histone H4 deacetylation [IDA, ISO]
- histone deacetylation [ISO]
- negative regulation by host of viral transcription [ISO]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IGI]
- negative regulation of androgen receptor signaling pathway [ISO]
- negative regulation of apoptotic process [IGI]
- negative regulation of canonical Wnt signaling pathway [IGI]
- negative regulation of cell proliferation [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of intrinsic apoptotic signaling pathway [IGI]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of peptidyl-lysine acetylation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- neuron differentiation [IGI]
- odontogenesis of dentin-containing tooth [IGI]
- positive regulation of cell proliferation [IGI, ISO]
- positive regulation of chemokine (C-X-C motif) ligand 2 production [ISO]
- positive regulation of interleukin-1 production [ISO]
- positive regulation of oligodendrocyte differentiation [IGI, ISO]
- positive regulation of receptor biosynthetic process [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of tumor necrosis factor production [ISO]
- positive regulation of type B pancreatic cell apoptotic process [ISO]
- positive regulation of tyrosine phosphorylation of Stat3 protein [ISO]
- protein deacetylation [ISO]
- response to amphetamine [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- Krueppel-associated box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [IPI, ISO]
- RNA polymerase II transcription corepressor activity [ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA, ISO]
- core promoter binding [IDA, ISO]
- deacetylase activity [IDA]
- enzyme binding [ISO]
- histone deacetylase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein deacetylase activity [ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [IDA]
- transcription factor binding [ISO, TAS]
- transcription regulatory region DNA binding [ISO]
- transcription regulatory region sequence-specific DNA binding [IDA]
- DNA binding [IDA]
- Krueppel-associated box domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- RNA polymerase II repressing transcription factor binding [IPI, ISO]
- RNA polymerase II transcription corepressor activity [ISO]
- activating transcription factor binding [ISO]
- chromatin binding [IDA, ISO]
- core promoter binding [IDA, ISO]
- deacetylase activity [IDA]
- enzyme binding [ISO]
- histone deacetylase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein deacetylase activity [ISO]
- repressing transcription factor binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [IDA]
- transcription factor binding [ISO, TAS]
- transcription regulatory region DNA binding [ISO]
- transcription regulatory region sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
- NuRD complex [IDA, IPI, ISO]
- Sin3 complex [ISO]
- chromatin [ISO]
- cytoplasm [TAS]
- cytosol [ISO]
- heterochromatin [IDA]
- histone deacetylase complex [IPI, TAS]
- neuron projection [ISO]
- neuronal cell body [IDA]
- nuclear chromatin [IDA, ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [IDA, ISO]
- transcription factor complex [IDA, IPI]
- transcriptional repressor complex [IPI]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Differentiated embryo chondrocyte 1 (DEC1) represses PPARγ2 gene through interacting with CCAAT/enhancer binding protein β (C/EBPβ).
DEC1 is a transcription repressor that is induced by Hypoxia-Inducible Factor-α/β (HIF-α/β). In this study, we found that either hypoxic treatment or ectopic expression of DEC1 blocks induction of a master adipogenic transactivator, peroxisome proliferative activated receptor-γ2 (PPARγ2) in 3T3-L1 cells. DEC1 did not prevent C/EBPβ, which is an upstream transactivator for PPARγ2, from occupying the PPARγ2 promoter. DEC1 occupied ... [more]
Mol. Cells Jun. 01, 2012; 33(6);575-81 [Pubmed: 22610404]
Throughput
- Low Throughput
Additional Notes
- figure 4D.
Curated By
- BioGRID