An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription.

Tsai YC, Greco TM, Boonmee A, Miteva Y, Cristea IM

Among mammalian sirtuins, SIRT7 is the only enzyme residing in nucleoli where ribosomal DNA is transcribed. Recent reports established that SIRT7 associates with RNA Pol I machinery and is required for rDNA transcription. Although defined by its homology to the yeast histone deacetylase Sir2, current knowledge suggests that SIRT7 itself has little to no deacetylase activity. Because only two SIRT7 ... [more]

Mol. Cell Proteomics May. 01, 2012; 11(5);60-76 [Pubmed: 22586326]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

exogenous expression of bait
figure 2, table S1.

Curated By

BioGRID

Interaction Summary

SIRT7

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD-dependent histone deacetylase activity (H3-K18 specific) [IDA]chromatin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

AHNAK

Gene Ontology Biological Process

Gene Ontology Molecular Function

S100 protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]structural molecule activity conferring elasticity [ISS]