BAIT

HHEX

HEX, HMPH, HOX11L-PEN, PRH, PRHX
hematopoietically expressed homeobox
GO Process (17)
GO Function (11)
GO Component (3)

Gene Ontology Cellular Component

Homo sapiens
PREY

PSMA3

HC8, PSC3
proteasome (prosome, macropain) subunit, alpha type, 3
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The transcriptional repressor protein PRH interacts with the proteasome.

Bess KL, Swingler TE, Rivett AJ, Gaston K, Jayaraman PS

PRH (proline-rich homeodomain protein)/Hex is important in the control of cell proliferation and differentiation. We have shown previously that PRH contains two domains that can bring about transcriptional repression independently; the PRH homeodomain represses transcription by binding to TATA box sequences, whereas the proline-rich N-terminal domain can repress transcription by interacting with members of the Groucho/TLE (transducin-like enhancer of split) ... [more]

Biochem. J. Sep. 15, 2003; 374(0);667-75 [Pubmed: 12826010]

Throughput

  • Low Throughput

Curated By

  • BioGRID