Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

PRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.

Illingworth RS, Botting CH, Grimes GR, Bickmore WA, Eskeland R

The essential histone variant H2A.Z localises to both active and silent chromatin sites. In embryonic stem cells (ESCs), H2A.Z is also reported to co-localise with polycomb repressive complex 2 (PRC2) at developmentally silenced genes. The mechanism of H2A.Z targeting is not clear, but a role for the PRC2 component Suz12 has been suggested. Given this association, we wished to determine ... [more]

PLoS ONE Apr. 13, 2012; 7(4);e34848 [Pubmed: 22496869]

Throughput

  • High Throughput

Additional Notes

  • figure 1, table S1.

Curated By

  • BioGRID