Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Consequences of COP9 signalosome and 26S proteasome interaction.

Huang X, Hetfeld BK, Seifert U, Kaehne T, Kloetzel PM, Naumann M, Bech-Otschir D, Dubiel W

The COP9 signalosome (CSN) occurs in all eukaryotic cells. It is a regulatory particle of the ubiquitin (Ub)/26S proteasome system. The eight subunits of the CSN possess sequence homologies with the polypeptides of the 26S proteasome lid complex and just like the lid, the CSN consists of six subunits with PCI (proteasome, COP9 signalosome, initiation factor 3) domains and two ... [more]

FEBS J. Aug. 01, 2005; 272(15);3909-17 [Pubmed: 16045761]

Throughput

  • Low Throughput

Curated By

  • BioGRID