BAIT

PSMC1

AI325227, P26s4, Rpt2/S4, S4, rpt2
protease (prosome, macropain) 26S subunit, ATPase 1
GO Process (0)
GO Function (2)
GO Component (8)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

NGLY1

1110002C09Rik, PNGase, Png1
N-glycanase 1
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Multiple modes of interaction of the deglycosylation enzyme, mouse peptide N-glycanase, with the proteasome.

Li G, Zhou X, Zhao G, Schindelin H, Lennarz WJ

Peptide N-glycanase (PNGase) is involved in the cleavage of oligosaccharide chains from misfolded glycoproteins that are destined for degradation by the proteasome. Earlier, a number of potential binding partners of mouse PNGase (mPNGase) were detected by using the yeast two-hybrid system. In the current study, an in vitro system was set up to investigate direct interactions between mPNGase and these ... [more]

Proc. Natl. Acad. Sci. U.S.A. Nov. 01, 2005; 102(44);15809-14 [Pubmed: 16249333]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NGLY1 PSMC1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID