PREY

UBB

ubiquitin B
GO Process (81)
GO Function (1)
GO Component (9)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions.

Lukic Z, Hausmann S, Sebastian S, Rucci J, Sastri J, Robia SL, Luban J, Campbell EM

The TRIM5 proteins are cellular restriction factors that prevent retroviral infection in a species-specific manner. Multiple experiments indicate that restriction activity requires accessory host factors, including E2-enzymes. To better understand the mechanism of restriction, we conducted yeast-two hybrid screens to identify proteins that bind to two TRIM5 orthologues.The only cDNAs that scored on repeat testing with both TRIM5 orthologues were ... [more]

Retrovirology Nov. 15, 2011; 8(0);93 [Pubmed: 22078707]

Throughput

  • Low Throughput

Additional Notes

  • #LPPI
  • Likely protein-protein interaction

Curated By

  • BioGRID