BAIT

PSMC3

proteasome (prosome, macropain) 26S subunit, ATPase 3
GO Process (1)
GO Function (1)
GO Component (7)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus
PREY

RNF19A

RNF19
ring finger protein 19A, RBR E3 ubiquitin protein ligase
UPS Project
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rnf19a, a ubiquitin protein ligase, and Psmc3, a component of the 26S proteasome, tether to the acrosome membranes and the head-tail coupling apparatus during rat spermatid development.

Rivkin E, Kierszenbaum AL, Gil M, Tres LL

We report the cDNA cloning of rat testis Rnf19a, a ubiquitin protein ligase, and show 98% and 93% protein sequence identity of testicular mouse and human Rnf19a, respectively. Rnf19a interacts with Psmc3, a protein component of the 19S regulatory cap of the 26S proteasome. During spermatid development, Rnf19a and Psmc3 are initially found in Golgi-derived proacrosomal vesicles. Later on, Rnf19a, ... [more]

Dev. Dyn. Jul. 01, 2009; 238(7);1851-61 [Pubmed: 19517565]

Throughput

  • Low Throughput

Curated By

  • BioGRID