SUZ12
Gene Ontology Biological Process
- histone H3-K27 trimethylation [ISO]
- histone methylation [IMP]
- histone ubiquitination [IMP]
- negative regulation of cell differentiation [ISO]
- negative regulation of chemokine production [ISO]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of tyrosine phosphorylation of Stat3 protein [ISO]
- positive regulation of cell proliferation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ESC/E(Z) complex [IDA, ISO]
- nucleolus [ISO]
- nucleus [IDA, ISO]
- sex chromatin [IDA]
EZH2
Gene Ontology Biological Process
- DNA methylation [IMP]
- G1 to G0 transition [IMP]
- cellular response to hydrogen peroxide [IDA]
- cerebellar cortex development [IMP]
- histone H3-K27 methylation [IMP, ISO]
- histone methylation [IDA]
- negative regulation of G1/S transition of mitotic cell cycle [IMP]
- negative regulation of epidermal cell differentiation [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of gene expression, epigenetic [ISO]
- negative regulation of retinoic acid receptor signaling pathway [ISO]
- negative regulation of striated muscle cell differentiation [IDA]
- negative regulation of transcription elongation from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of Ras GTPase activity [ISO]
- positive regulation of dendrite development [ISO]
- positive regulation of epithelial to mesenchymal transition [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- protein localization to chromatin [IMP]
- regulation of cell proliferation [IMP]
- regulation of circadian rhythm [IMP, ISO]
- regulation of gene expression [IMP]
- regulation of gliogenesis [IMP]
- regulation of neurogenesis [IMP]
- regulation of protein phosphorylation [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.
Polycomb repressive complex 2 (PRC2) trimethylates lysine 27 of histone H3 (H3K27me3) to regulate gene expression during diverse biological transitions in development, embryonic stem cell (ESC) differentiation, and cancer. Here, we show that Polycomb-like 3 (Pcl3) is a component of PRC2 that promotes ESC self-renewal. Using mass spectrometry, we identified Pcl3 as a Suz12 binding partner and confirmed Pcl3 interactions ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 1B.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EZH2 SUZ12 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| EZH2 SUZ12 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SUZ12 EZH2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 669475 | |
| SUZ12 EZH2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EZH2 SUZ12 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SUZ12 EZH2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EZH2 SUZ12 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EZH2 SUZ12 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | - | |
| SUZ12 EZH2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID