A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Polynucleotide kinase/phosphatase, Pnk1, is involved in base excision repair in Schizosaccharomyces pombe.

Kashkina E, Qi T, Weinfeld M, Young D

We previously reported that Schizosaccharomyces pombe pnk1 cells are more sensitive than wild-type cells to Î³-radiation and camptothecin, indicating that Pnk1 is required for DNA repair. Here, we report that pnk1pku70 and pnk1rhp51 double mutants are more sensitive to Î³-radiation than single mutants, from which we infer that Pnk1's primary role is independent of either homologous recombination or non-homologous end ... [more]

Unknown Jun. 27, 2012; 0(0); [Pubmed: 22748672]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
APN2 PNK1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Ryan CJ (2012)	High	-8.2989	BioGRID	761801

Curated By

BioGRID

Interaction Summary

PNK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]polynucleotide 3'-phosphatase activity [IDA, IMP]

Gene Ontology Cellular Component

APN2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-(apurinic or apyrimidinic site) lyase activity [IDA]double-stranded DNA 3'-5' exodeoxyribonuclease activity [IMP]phosphodiesterase I activity [IDA, ISO]