BAIT

BIRC2

API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1
baculoviral IAP repeat containing 2
GO Process (31)
GO Function (6)
GO Component (6)

Gene Ontology Biological Process

Homo sapiens
PREY

SH3KBP1

CD2BP3, CIN85, GIG10, HSB-1, HSB1, MIG18, RP11-345L8.1
SH3-domain kinase binding protein 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CIN85 associates with TNF receptor 1 via Src and modulates TNF-alpha-induced apoptosis.

Narita T, Nishimura T, Yoshizaki K, Taniyama T

CIN85 is a multidomain protein that associates with receptors carrying tyrosine kinase domains. Here we report that it is also a component of the signaling complex associated with tumor necrosis factor receptor 1 (TNFR1), which lacks a tyrosine kinase domain. This was established by showing that CIN85 was co-precipitated with TNFR1, TRADD, cIAP-1 and TARF1/2, but not with FADD, RIP, ... [more]

Exp. Cell Res. Mar. 10, 2005; 304(1);256-64 [Pubmed: 15707590]

Throughput

  • Low Throughput

Curated By

  • BioGRID