An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis.

Fukuda T, Pratto F, Schimenti JC, Turner JM, Camerini-Otero RD, Hoeoeg C

Meiotic recombination and chromosome synapsis between homologous chromosomes are essential for proper chromosome segregation at the first meiotic division. While recombination and synapsis, as well as checkpoints that monitor these two events, take place in the context of a prophase I-specific axial chromosome structure, it remains unclear how chromosome axis components contribute to these processes. We show here that many ... [more]

PLoS Genet. Feb. 01, 2012; 8(2);e1002485 [Pubmed: 22346761]

Throughput

Low Throughput

Additional Notes

figure 3. Smc3 was pulled down by Ser1083-Pi specific antibody.

Curated By

BioGRID

Interaction Summary

SMC3

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]dynein binding [ISO]mediator complex binding [IDA]protein binding [IPI]protein heterodimerization activity [ISO]

Gene Ontology Cellular Component

HORMAD1