An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Vezf1 protein binding sites genome-wide are associated with pausing of elongating RNA polymerase II.

Gowher H, Brick K, Camerini-Otero RD, Felsenfeld G

The protein Vezf1 plays multiple roles important for embryonic development. In Vezf1(-/-) mouse embryonic stem (mES) cells, our earlier data showed widespread changes in gene-expression profiles, including decreased expression of the full-length active isoform of Dnmt3b methyltransferase and concomitant genome-wide reduction in DNA methylation. Here we show that in HeLaS3 cells there is a strong genome-wide correlation between Vezf1 binding ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 14, 2012; 109(7);2370-5 [Pubmed: 22308494]

Throughput

Low Throughput

Additional Notes

figure S10

Curated By

BioGRID

Interaction Summary

VEZF1

Gene Ontology Biological Process

Gene Ontology Cellular Component

TOP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISO]DNA topoisomerase type I activity [IDA, ISO]chromatin DNA binding [ISO]chromatin binding [ISO]core promoter sequence-specific DNA binding [ISO]poly(A) RNA binding [ISO]protein complex binding [ISO]