BAIT

AT5G15240

F8M21.130, F8M21_130
transmembrane amino acid transporter family protein
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ABCB19

ATABCB19, ATMDR1, ATMDR11, ATP BINDING CASSETTE SUBFAMILY B19, ATPGP19, MDR1, MDR11, MULTIDRUG RESISTANCE PROTEIN 11, P-GLYCOPROTEIN 19, PGP19, AT3G28860
ABC transporter B family member 19
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Uncovering Arabidopsis membrane protein interactome enriched in transporters using mating-based split ubiquitin assays and classification models.

Chen J, Lalonde S, Obrdlik P, Noorani Vatani A, Parsa SA, Vilarino C, Revuelta JL, Frommer WB, Rhee SY

High-throughput data are a double-edged sword; for the benefit of large amount of data, there is an associated cost of noise. To increase reliability and scalability of high-throughput protein interaction data generation, we tested the efficacy of classification to enrich potential protein-protein interactions. We applied this method to identify interactions among Arabidopsis membrane proteins enriched in transporters. We validated our ... [more]

Front Plant Sci Jun. 28, 2012; 3(0);124 [Pubmed: 22737156]

Throughput

  • High Throughput

Curated By

  • BioGRID