An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

LINC complexes form by binding of three KASH peptides to domain interfaces of trimeric SUN proteins.

Sosa BA, Rothballer A, Kutay U, Schwartz TU

Linker of nucleoskeleton and cytoskeleton (LINC) complexes span the nuclear envelope and are composed of KASH and SUN proteins residing in the outer and inner nuclear membrane, respectively. LINC formation relies on direct binding of KASH and SUN in the perinuclear space. Thereby, molecular tethers are formed that can transmit forces for chromosome movements, nuclear migration, and anchorage. We present ... [more]

Cell May. 25, 2012; 149(5);1035-47 [Pubmed: 22632968]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SYNE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IDA]actin filament binding [ISS]lamin binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [ISS]

Gene Ontology Cellular Component

SUN1