BAIT
GCK
FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2
glucokinase (hexokinase 4)
GO Process (17)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular glucose homeostasis [IBA]
- cellular response to insulin stimulus [ISS]
- cellular response to leptin stimulus [ISS]
- detection of glucose [IMP]
- endocrine pancreas development [TAS]
- glucose homeostasis [IMP]
- glucose transport [TAS]
- hexose transport [TAS]
- negative regulation of gluconeogenesis [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of insulin secretion [IMP]
- regulation of glucose transport [TAS]
- regulation of glycolytic process [NAS]
- regulation of insulin secretion [IMP]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Homo sapiens
PREY
PFKFB2
PFK-2/FBPase-2, RP11-164O23.2
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
GO Process (5)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel interaction partner.
The low affinity glucose-phosphorylating enzyme glucokinase shows the phenomenon of intracellular translocation in beta cells of the pancreas and the liver. To identify potential binding partners of glucokinase by a systematic strategy, human beta cell glucokinase was screened by a 12-mer random peptide library displayed by the M13 phage. This panning procedure revealed two consensus motifs with a high binding ... [more]
J. Biol. Chem. Nov. 23, 2001; 276(47);43915-23 [Pubmed: 11522786]
Throughput
- Low Throughput
Curated By
- BioGRID