PREY

PFKFB2

PFK-2/FBPase-2, RP11-164O23.2
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
GO Process (5)
GO Function (3)
GO Component (1)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel interaction partner.

Baltrusch S, Lenzen S, Okar DA, Lange AJ, Tiedge M

The low affinity glucose-phosphorylating enzyme glucokinase shows the phenomenon of intracellular translocation in beta cells of the pancreas and the liver. To identify potential binding partners of glucokinase by a systematic strategy, human beta cell glucokinase was screened by a 12-mer random peptide library displayed by the M13 phage. This panning procedure revealed two consensus motifs with a high binding ... [more]

J. Biol. Chem. Nov. 23, 2001; 276(47);43915-23 [Pubmed: 11522786]

Throughput

  • Low Throughput

Curated By

  • BioGRID