An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The novel function of HINFP as a co-activator in sterol-regulated transcription of PCSK9 in HepG2 cells.

Li H, Liu J

PCSK9 (proprotein convertase subtilisin/kexin typeÂ 9) plays an important role in control of plasma LDL (low-density lipoprotein) cholesterol metabolism by modulating the degradation of hepatic LDL receptor. Previous studies demonstrated that PCSK9 is a target gene of the SREBP2 [SRE (sterol-regulatory element)-binding protein 2] that activates PCSK9 gene transcription through an SRE motif of the promoter. In addition to SREBP2, HNF1Î± ... [more]

Biochem. J. May. 01, 2012; 443(3);757-68 [Pubmed: 22288532]

Throughput

Low Throughput

Additional Notes

figure 4B.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NPAT TRRAP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	DeRan M (2008)	Low	-	BioGRID	-
NPAT TRRAP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Li H (2012)	Low	-	BioGRID	672216
TRRAP NPAT	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	DeRan M (2008)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TRRAP

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [IBA]protein binding [IPI]transcription cofactor activity [IDA]

Gene Ontology Cellular Component

NPAT

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein C-terminus binding [IPI]protein N-terminus binding [IPI]protein binding [IPI]transcription coactivator activity [IDA, IMP]transcription corepressor activity [IMP]