Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Decoding the intrinsic mechanism that prohibits ALIX interaction with ESCRT and viral proteins.

Zhou X, Si J, Corvera J, Gallick GE, Kuang J

The adaptor protein ALIX [ALG-2 (apoptosis-linked-gene-2 product)-interacting protein X] links retroviruses to ESCRT (endosomal sorting complex required for transport) machinery during retroviral budding. This function of ALIX requires its interaction with the ESCRT-III component CHMP4 (charged multivesicular body protein 4) at the N-terminal Bro1 domain and retroviral Gag proteins at the middle V domain. Since cytoplasmic or recombinant ALIX is ... [more]

Biochem. J. Dec. 15, 2010; 432(3);525-34 [Pubmed: 20929444]

Throughput

  • Low Throughput

Curated By

  • BioGRID