BAIT

SZY-2

Y32H12A.4, CELE_Y32H12A.4

szy-2 encodes an ortholog of various protein phosphatase 1 (PP1) regulatory (inhibitor) subunit 2 proteins; the phosphatase inhibitory activity of recombinant SZY-2 has been validated in vitro, with an IC50 of 5 nM versus purified PP1 catalytic subunit; SZY-2 has anomalously low mobility in SDS-PAGE, consistent with the hypothesis that it (like its orthologs) is a naturally disordered protein that uses native flexibility to bind its substrates; SZY-2 is efficiently phosphorylated in vitro by casein kinase II or CDC2/cyclinB, but not by glycogen synthase kinase-3; szy-2 is required for embryonic development in mass RNAi assays.

GO Process (0)

GO Function (0)

GO Component (0)

WormBase Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Caenorhabditis elegans

PREY

KAP-1

CELE_F08F8.3

Protein KAP-1

GO Process (2)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

metabolic process [IDA]

microtubule-based movement [IDA]

Gene Ontology Molecular Function

microtubule motor activity [IDA]

Gene Ontology Cellular Component

axonemal heterotrimeric kinesin-II complex [IPI]

ciliary transition zone [IDA]

nonmotile primary cilium [IDA]

WormBase Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A map of the interactome network of the metazoan C. elegans.

Li S, Armstrong CM, Bertin N, Ge H, Milstein S, Boxem M, Vidalain PO, Han JD, Chesneau A, Hao T, Goldberg DS, Li N, Martinez M, Rual JF, Lamesch P, Xu L, Tewari M, Wong SL, Zhang LV, Berriz GF, Jacotot L, Vaglio P, Reboul J, Hirozane-Kishikawa T, Li Q, Gabel HW, Elewa A, Baumgartner B, Rose DJ, Yu H, Bosak S, Sequerra R, Fraser A, Mango SE, Saxton WM, Strome S, Van Den Heuvel S, Piano F, Vandenhaute J, Sardet C, Gerstein M, Doucette-Stamm L, Gunsalus KC, Harper JW, Cusick ME, Roth FP, Hill DE, Vidal M

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together ... [more]

Science Jan. 23, 2004; 303(5657);540-3 [Pubmed: 14704431]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary