BAIT

BHLHE40

BHLHB2, DEC1, HLHB2, SHARP-2, STRA13, Stra14
basic helix-loop-helix family, member e40
Homo sapiens
PREY

STAT3

ADMIO, APRF, HIES
signal transducer and activator of transcription 3 (acute-phase response factor)
GO Process (27)
GO Function (10)
GO Component (5)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

STRA13 interacts with STAT3 and modulates transcription of STAT3-dependent targets.

Ivanova AV, Ivanov SV, Zhang X, Ivanov VN, Timofeeva OA, Lerman MI

STRA13 is a pVHL-dependent bHLH transcription factor up-regulated on the mRNA level in multiple cancer cell lines and implicated recently in the regulation of immune cell homeostasis and autoimmunity. In searching for STRA13-interacting proteins with oncogenic potential by the yeast two-hybrid screening, we identified STAT3 beta as a STRA13-binding partner. We showed that STRA13 binds predominantly to phosphorylated (active) STAT3 ... [more]

J. Mol. Biol. Jul. 16, 2004; 340(4);641-53 [Pubmed: 15223310]

Throughput

  • Low Throughput

Curated By

  • BioGRID