BAIT

KDM6A

KABUK2, UTX, bA386N14.2, RP13-886N14.3
lysine (K)-specific demethylase 6A
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

UTX, a histone H3-lysine 27 demethylase, acts as a critical switch to activate the cardiac developmental program.

Lee S, Lee JW, Lee SK

The removal of histone H3 lysine27 (H3K27) trimethylation mark is important for the robust induction of many cell type-specific genes during differentiation. Here we show that UTX, a H3K27 demethylase, acts as a critical switch to promote a cardiac-specific gene program. UTX-deficient ESCs failed to develop heart-like rhythmic contractions under a cardiac differentiation condition. UTX-deficient mice show severe defects in ... [more]

Dev. Cell Jan. 17, 2012; 22(1);25-37 [Pubmed: 22192413]

Throughput

  • Low Throughput

Additional Notes

  • figure 4. The transcriptional activity of SRF, Tbx5, and Nkx2.5, alone or in combination, was enhanced by UTX

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TBX5 KDM6A
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
673871

Curated By

  • BioGRID