KDM6A
Gene Ontology Biological Process
Gene Ontology Cellular Component
NKX2-5
Gene Ontology Biological Process
- adult heart development [IMP]
- atrial cardiac muscle cell development [ISS]
- atrial septum morphogenesis [IMP]
- cardiac conduction system development [IMP]
- cardiac muscle cell differentiation [ISS]
- cardiac muscle tissue morphogenesis [IMP]
- cell differentiation [ISS]
- embryonic heart tube development [ISS]
- heart looping [ISS]
- heart morphogenesis [ISS]
- hemopoiesis [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of canonical Wnt signaling pathway [ISS]
- negative regulation of cardiac muscle cell apoptotic process [IMP]
- negative regulation of myotube differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [ISS]
- outflow tract septum morphogenesis [IMP]
- pharyngeal system development [ISS]
- positive regulation of cardioblast differentiation [ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of heart contraction [ISS]
- positive regulation of neuron differentiation [IMP]
- positive regulation of sodium ion transport [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISS]
- positive regulation of transcription initiation from RNA polymerase II promoter [ISS]
- positive regulation of transcription via serum response element binding [ISS]
- positive regulation of transcription, DNA-templated [IDA, ISS]
- positive regulation of voltage-gated calcium channel activity [ISS]
- regulation of cardiac muscle contraction [ISS]
- right ventricular cardiac muscle tissue morphogenesis [IMP]
- septum secundum development [IMP]
- spleen development [IMP, ISS]
- thyroid gland development [IMP]
- vasculogenesis [ISS]
- ventricular cardiac muscle cell development [ISS]
- ventricular septum morphogenesis [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- chromatin binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [ISS]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- serum response element binding [ISS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- chromatin binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [ISS]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- serum response element binding [ISS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
UTX, a histone H3-lysine 27 demethylase, acts as a critical switch to activate the cardiac developmental program.
The removal of histone H3 lysine27 (H3K27) trimethylation mark is important for the robust induction of many cell type-specific genes during differentiation. Here we show that UTX, a H3K27 demethylase, acts as a critical switch to promote a cardiac-specific gene program. UTX-deficient ESCs failed to develop heart-like rhythmic contractions under a cardiac differentiation condition. UTX-deficient mice show severe defects in ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4. The transcriptional activity of SRF, Tbx5, and Nkx2.5, alone or in combination, was enhanced by UTX
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KDM6A NKX2-5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 673872 | |
| NKX2-5 KDM6A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 673874 |
Curated By
- BioGRID