An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Co-chaperone CHIP promotes aggregation of ataxin-1.

Choi JY, Ryu JH, Kim HS, Park SG, Bae KH, Kang S, Myung PK, Cho S, Park BC, Lee do H

Recent studies demonstrated that co-chaperone/E3 ligase CHIP (C-terminus of hsp70-interacting protein) mediates the ubiquitylation and suppresses the aggregation of polyglutamine (polyQ) proteins, such as huntingtin or ataxin-3. In this study, we investigated the effects of CHIP on the degradation of another polyQ protein ataxin-1. Interestingly CHIP associates not only with the polyQ-expanded ataxin-1 but also with the normal ataxin-1. Moreover, ... [more]

Mol. Cell. Neurosci. Jan. 01, 2007; 34(1);69-79 [Pubmed: 17127076]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ATXN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(G) binding [IDA]poly(U) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]protein self-association [IDA]

Gene Ontology Cellular Component

HSPA4